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4 6 diamidine 2 phenylindole  (Thermo Fisher)


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    Structured Review

    Thermo Fisher 4 6 diamidine 2 phenylindole
    In vitro-seeded cells on thermoplastic polyurethane (TPU) vs. CTRL. (A) Scanning electron microscopy (SEM) analysis of in vitro-seeded cells on TPU specimens vs. glass (CTRL). Scale bar = 20 μm. (B to D) Immunofluorescence (IF) staining showing the expression of cell-specific markers. PVAT, perivascular adipose tissue (PVAT)-derived cells; ECs, endothelial cells; SMCs, smooth <t>muscle</t> <t>cells.</t> <t>4′,6-Diamidine-2-phenylindole</t> (DAPI) = nuclei. Scale bar = 50 μm.
    4 6 Diamidine 2 Phenylindole, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/diamidine/4'%2C6'-Diamidino-2-phenylindole+dihydrochloride%2C+98%25/pmc13213075-87-15-17
    Average 99 stars, based on 1 article reviews
    4 6 diamidine 2 phenylindole - by Bioz Stars, 2026-09
    99/100 stars

    Images

    1) Product Images from "Exploring Perivascular Adipose Tissue Responses to Bioresorbable Thermoplastic Polyurethane Vascular Grafts"

    Article Title: Exploring Perivascular Adipose Tissue Responses to Bioresorbable Thermoplastic Polyurethane Vascular Grafts

    Journal: Biomaterials Research

    doi: 10.34133/bmr.0372

    In vitro-seeded cells on thermoplastic polyurethane (TPU) vs. CTRL. (A) Scanning electron microscopy (SEM) analysis of in vitro-seeded cells on TPU specimens vs. glass (CTRL). Scale bar = 20 μm. (B to D) Immunofluorescence (IF) staining showing the expression of cell-specific markers. PVAT, perivascular adipose tissue (PVAT)-derived cells; ECs, endothelial cells; SMCs, smooth muscle cells. 4′,6-Diamidine-2-phenylindole (DAPI) = nuclei. Scale bar = 50 μm.
    Figure Legend Snippet: In vitro-seeded cells on thermoplastic polyurethane (TPU) vs. CTRL. (A) Scanning electron microscopy (SEM) analysis of in vitro-seeded cells on TPU specimens vs. glass (CTRL). Scale bar = 20 μm. (B to D) Immunofluorescence (IF) staining showing the expression of cell-specific markers. PVAT, perivascular adipose tissue (PVAT)-derived cells; ECs, endothelial cells; SMCs, smooth muscle cells. 4′,6-Diamidine-2-phenylindole (DAPI) = nuclei. Scale bar = 50 μm.

    Techniques Used: In Vitro, Electron Microscopy, Immunofluorescence, Staining, Expressing, Derivative Assay

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    Staining:

    Article Title: Allium cepa: A Natural Enhancer of Wound Closure and Cell Viability in O-Carboxymethyl Chitosan Films
    Article Snippet: .. After culturing the cells for up to 72 hours, the cell nuclei were stained blue by incubating them in a PBS solution containing 4′,6-Diamidine-2′-phenylindole dihydrochloride (DAPI) (InvitrogenTM, Thermo Fisher Scientific, Waltham, MA, USA). ..

    Article Title: Prime-Target neoantigen vaccination unleashes unprecedented T cell immunity within “cold” immunosuppressive tumors
    Article Snippet: .. Sections were then stained with antibody solution containing 3× SSC buffer (Sigma-Aldrich), 2% Bovine Serum Albumin (BSA, Sigma-Aldrich), 4′,6-Diamidine-2′-phenylindole dihydrochloride (DAPI, Invitrogen) and the antibodies listed in Supplementary Table 2 for 1 h at room temperature. .. After washing with wash buffer (3× SSC buffer and 2% BSA), stained sections were mounted using ProLongTM Gold Antifade Mountant (Invitrogen).

    Article Title: Development of low-cost electrical stimulation device to promote hiPSC-CM differentiation and functionality
    Article Snippet: Cells were then washed with blocking buffer and incubated with anti-rabbit Alexa-488 secondary antibody in blocking buffer (1:400, Sigma-Aldrich, SAB4600044). .. Cells were co-stained with nuclear Draq5 (Cell Signal, 4048L) or 4′, 6-Diamidine-2′-phenylindole dihydrochloride (DAPI; Thermo Fisher, D1306) stain. .. Stained samples were imaged using an Olympus FV3000 Confocal Laser Scanning Microscope.

    Article Title: Optimal design for multi-compartment cell elasticity estimation using AFM and super-resolution imaging.
    Article Snippet: Cells were then stained in a two-step process: (i) samples were incubated with mouse anti-Lamin-A (Invitrogen) and rabbit anti-paxillin (phosphoY31, Abcam); (ii) secondary staining was performed with donkey anti-rabbit Alexa Fluor 555 (Invitrogen Molecular Probes, Thermo Fisher Scientific) and donkey anti-mouse Alexa Fluor 647 (Invitrogen Molecular Probes, Thermo Fisher Scientific). .. Finally, actin and DNA were stained with phalloidin Alexa Fluor 488 (Invitrogen Molecular Probes, Thermo Fisher Scientific) and 4’,6’-diamidine-2’-phenylindole dihydrochloride (DAPI, Roche Diagnostics), respectively. ..

    Article Title: Rutin Attenuates Virus Entry and Replication and Exerts Neuroprotection in Experimental Models of Japanese Encephalitis.
    Article Snippet: (prM), and Envelope (E) and seven non-structural proteins (NS1 to NS5) (Sumiyoshi et al. 1987; Chambers et al. 1990) by the host and the viral proteases (Joe et al. 2022).. The structural proteins are essential for virion assembly and infectivity (Harrison 2008), while the non-structural proteins contribute to the life cycle of the virus, including RNA replication, virus assembly, and evasion of host innate immunity (Chambers et al. 1990; Kumar et al. 2022).. The ectodomain of the envelope protein exists as a homodimer folded into three distinct domains: domain I (DI), a central β-barrel region; domain II (DII), a finger-like dimerization region with a fusion loop; and domain III (DIII) that contains cellular receptor-binding motifs (Luca et al. 2012).

    CCK-8 Assay:

    Article Title: Development of a KRP-based pH-responsive drug delivery system for solid tumors
    Article Snippet: Our cell culture media [Dulbecco’s Modified Eagle Medium (DMEM), F12] were acquired from Sigma-Aldrich (St. Louis, MO, USA), while fetal bovine serum (FBS) was sourced from Novagen (Madison, WI, USA). .. The Cell Counting Kit-8 (CCK-8) assay was obtained from Dojindo Laboratories (Kumamoto, Japan), and LysoTracker Green and 4',6-diamidine-2'-phenylindole dihydrochloride (DAPI) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). .. DOX and DOX hydrochloride (DOX-HCL) were supplied by Hisun Pharmaceutical Co. (Taizhou, China).

    Incubation:

    Article Title: qChIP-MS reveals the local chromatin composition by label-free quantitative proteomics.
    Article Snippet: Coverslips were again washed three times with blocking buffer for 5 min each and once with PBS, and mounted on glass slides with ProLong Gold Antifade Mountant with DAPI (Thermo Scientific). .. Alternatively, Coverslips were/will be next incubated with 2mg/mL 4′,6-diamidine-2′-phenylindole dihydrochloride (DAPI) diluted 1:1,000 in blocking buffer for 5 min and washed again three times with blocking buffer for 3 min each and mounted on glass slides with ProLong Gold Antifade Mountant (Thermo Scientific). ..

    Blocking Assay:

    Article Title: qChIP-MS reveals the local chromatin composition by label-free quantitative proteomics.
    Article Snippet: Coverslips were again washed three times with blocking buffer for 5 min each and once with PBS, and mounted on glass slides with ProLong Gold Antifade Mountant with DAPI (Thermo Scientific). .. Alternatively, Coverslips were/will be next incubated with 2mg/mL 4′,6-diamidine-2′-phenylindole dihydrochloride (DAPI) diluted 1:1,000 in blocking buffer for 5 min and washed again three times with blocking buffer for 3 min each and mounted on glass slides with ProLong Gold Antifade Mountant (Thermo Scientific). ..



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    In vitro-seeded cells on thermoplastic polyurethane (TPU) vs. CTRL. (A) Scanning electron microscopy (SEM) analysis of in vitro-seeded cells on TPU specimens vs. glass (CTRL). Scale bar = 20 μm. (B to D) Immunofluorescence (IF) staining showing the expression of cell-specific markers. PVAT, perivascular adipose tissue (PVAT)-derived cells; ECs, endothelial cells; SMCs, smooth muscle cells. 4′,6-Diamidine-2-phenylindole (DAPI) = nuclei. Scale bar = 50 μm.

    Journal: Biomaterials Research

    Article Title: Exploring Perivascular Adipose Tissue Responses to Bioresorbable Thermoplastic Polyurethane Vascular Grafts

    doi: 10.34133/bmr.0372

    Figure Lengend Snippet: In vitro-seeded cells on thermoplastic polyurethane (TPU) vs. CTRL. (A) Scanning electron microscopy (SEM) analysis of in vitro-seeded cells on TPU specimens vs. glass (CTRL). Scale bar = 20 μm. (B to D) Immunofluorescence (IF) staining showing the expression of cell-specific markers. PVAT, perivascular adipose tissue (PVAT)-derived cells; ECs, endothelial cells; SMCs, smooth muscle cells. 4′,6-Diamidine-2-phenylindole (DAPI) = nuclei. Scale bar = 50 μm.

    Article Snippet: Subsequently, samples were again washed with PBS/1% BSA, placed on microscopy slides, and embedded in 4′,6-diamidine-2-phenylindole (DAPI; Thermo Fisher Scientific, Austria) containing a mounting medium (Agilent Technologies, Austria).

    Techniques: In Vitro, Electron Microscopy, Immunofluorescence, Staining, Expressing, Derivative Assay

    The S100B inhibitor pentamidine in combination with BEV improves the ovarian cancer response compared to BEV alone. (A) Flowchart of the in vivo experiment. Two weeks after intraperitoneal inoculation of ovarian cancer cells, drug treatment was administered. The mice were randomly divided into a normal saline treatment control group, BEV treatment group, pentamidine treatment group, and BEV with pentamidine treatment group. (B) Line chart of the tumor fluorescence intensity of the four groups of mice. (C) Tumor fluorescence imaging of mice after tumor formation (week 2), after killing the control group and pentamidine treatment group (week 4–6), after killing the BEV treatment group (week 8–9), and after killing the BEV with pentamidine treatment group (week 13–14). (D) Kaplan–Meier survival curves of the four groups of mice. (E) CD31 immunohistochemical staining and (F) MVD statistics of tumor tissue from the four groups of mice. (E) S100B immunohistochemical staining and S100B (G) staining intensity of tumor tissue from the four groups of mice. BEV: Bevacizumab; MVD: Micro-vessel density; *: P < 0.05; **: P < 0.01;^: P < 0.05 vs. NC; #: P < 0.05 vs. pentamidine; &: P < 0.05 vs. BEV.

    Journal: Journal of Advanced Research

    Article Title: S100B induces angiogenesis via the clathrin/FOXO1/β-catenin signaling pathway and contributes to Bevacizumab resistance in epithelial ovarian cancer

    doi: 10.1016/j.jare.2025.05.060

    Figure Lengend Snippet: The S100B inhibitor pentamidine in combination with BEV improves the ovarian cancer response compared to BEV alone. (A) Flowchart of the in vivo experiment. Two weeks after intraperitoneal inoculation of ovarian cancer cells, drug treatment was administered. The mice were randomly divided into a normal saline treatment control group, BEV treatment group, pentamidine treatment group, and BEV with pentamidine treatment group. (B) Line chart of the tumor fluorescence intensity of the four groups of mice. (C) Tumor fluorescence imaging of mice after tumor formation (week 2), after killing the control group and pentamidine treatment group (week 4–6), after killing the BEV treatment group (week 8–9), and after killing the BEV with pentamidine treatment group (week 13–14). (D) Kaplan–Meier survival curves of the four groups of mice. (E) CD31 immunohistochemical staining and (F) MVD statistics of tumor tissue from the four groups of mice. (E) S100B immunohistochemical staining and S100B (G) staining intensity of tumor tissue from the four groups of mice. BEV: Bevacizumab; MVD: Micro-vessel density; *: P < 0.05; **: P < 0.01;^: P < 0.05 vs. NC; #: P < 0.05 vs. pentamidine; &: P < 0.05 vs. BEV.

    Article Snippet: The pentamidine treatment group was administered pentamidine (Selleck, China) at a dosage of 10 mg/kg intraperitoneally three times a week [ ].

    Techniques: In Vivo, Saline, Control, Fluorescence, Imaging, Immunohistochemical staining, Staining